Wednesday, February 27, 2013

Determination Of Quinine In Urine By Fluorescence Spectroscopy

DETERMINATION OF QUININE IN URINE BY FLUORESCENCE SPECTROSCOPY
AT capital of the United Kingdom SOUTH BANK’S UNIVERSITY’S LABORATORY

INSTRUMENTAL METHODS OF analysis 2006

FORENSIC SCIENCE BSC

Determination of quinine in urine by fluorescence spectroscopy.

This experiment was carried out by ........... and .......... at London southernmost Bank University’s laboratory on the 31/10/2006.

Summery
How to define for quinine in an unknown urine sample containing roughly 100 μg cm-3 with the use of standard addition in fluorescence spectroscopy. The result obtained for this experiment was 85.48 μg cm-3 which is close to the estimated 100 μg cm-3.

CONTENTSPAGE

1.0INTRODUCTION...……………..………………………………………........4-8
1.1Theory 4
1.1.1Why do we need to quantify for quinine in urine? 5 1.1.2Why does quinine show fluorescence? 5 1.1.3How stable is quinine? 5
1.1.4Why is pH important? 5
1.1.5What are the advantages in using its fluorescence rather than its absorbance to quantify? 6
1.1.

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6Do we need to melt out any sample preparation or plum up stages? 7
1.1.7Are there any sources of error or interference? 7
1.1.8The advantages of standard addition as compared to external standardisation 7
1.2Aim 8

2.0METHOD……………………………………………………………………9-12
2.1Apparatus 9
2.2Chemicals 9
2.3Safety consideration 10
2.4 action 11-12

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