HELEN
PROFESSOR BUELL
BIO 101L-03
11/22/11
The effects of salt concentration on an enzyme catalyzed reaction
INTRODUCTION
This examine was performed to investigate how the physical factors of table salt
will affect the roll of an enzyme catalyzed reaction. (How fast an enzyme will turn
over a substrate, establish on energy of activation.) We tested on various levels of table salt
concentration, and used catechol to represent substrate, and white potato vine extract to represent
enzyme. We unploughed the levels of the substrate and enzyme at a constant, in an effort
to exactly test on the salinity concentration.
If our hypothesis is supported, our results will verbalise that the higher concentration of salt, the faster the enzyme will denature.
MATERIALS AND METHODS
* 5 mL of potato extract
* 10 mL of catechol
* 3 mL of .1% salinity concentration
* 3 mL of .
9% salinity concentration
* 3 mL of 2% salinity concentration
* 3 mL of 5% salinity concentration
* 3 mL of 20% salinity concentration
* 1- 5 mL pipettes
* Pi-pump for 5 mL pipette
* Wax marker
* 5 test subways
* Test resistance rack
My partner and I placed 1 mL of potato extract in five test tubes, on with 2
mL of catechol, to that we added 3 mL of .1% salinity concentration in tube one, 3 mL
of .9% salinity concentration in tube two, 2% salinity concentration in tube three, 3 mL
of 5% salinity concentration in tube four, and lastly 3 mL of 20% salinity concentration
in tube five. Pour each potpourri in its own spec 20 tube, along with a separate spec 20
tube alter with only potato extract to use as a blank on the...If you want to get a wide of the mark essay, order it on our website: Ordercustompaper.com
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